International Journal of Farmacia International Journal of Farmacia

ISSN: 2455-8109

Search form

Articles

  1. Phytochemical screening and estimation of sun protection factor from fruits and plantsDownload Article

    Syeda Habeeba Humera, Qudsiya Fatima, Tehmina Naaz, P.Sandhya

    Abstract

    close

    The aim of the present study is phytochemical screening and the ultraviolet absorption properties of ethanolic herbal extracts of some commonly used fruits and vegetable sources by determining the sun protection factor (SPF) number. The invitro SPF number is determined according to the spectrophotomertic method for ethanolic herbal extracts after dilution with alcoholic solutions. The absorbances were recorded between 290-320 nm using UV-visible spectrophotometry. It was observed that all of the ethanolic herbal extract showed some UV protection capability.

  2. Development and validation of stability indicating RP-HPLC method for estimation of sofosbuvir in bulk and tablet dosage formDownload Article

    Jeyabaskaran M, Prof. Rambabu C, Yasmin Sulthana

    Abstract

    close

    A simple, precise and accurate stability indicating RP-HPLC method has been developed and subsequently validated for estimation of Sofosbuvir (SFB) in bulk and from their combination dosage form using a Kromasil C18 (250 mm × 4.6 mm, 5µm) at 250C. Mobile phase consisted mixture of 0.1% Ortho phosphoric acid buffer and acetonitrile in the ratio 55:45 (v/v) with flow rate was 1 ml/min and detection was carried out by photodiode array detector at 259nm. The retention time for SFB was found to be 2.069min. SFB and their dosage form were exposed to thermal, photolytic, oxidative, acid-base hydrolytic stress conditions, the stressed samples were analyzed by the proposed method. Peak purity results suggested no other co-eluting, interfering peaks from excipients, impurities, or degradation products due to variable stress condition, and the method is specific for the estimation of SFB in presence of their degradation products and impurities. The proposed method has permitted the quantification of SOF over linearity in the range of  0.1– 0.6 mg/ml and its percentage recovery was found to be  100.39 %. The % RSD of intraday and inter day precision were found 0.34% and 0.6% according to International Conference on Harmonization (ICH) Q2B guidelines.

Pages