International Journal of Farmacia International Journal of Farmacia

ISSN: 2455-8109

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  1. Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Sofosbuvir in Bulk and Tablet Dosage FormDownload Article

    Jeyabaskaran M, Prof.Rambabu C, Yasmin Sulthana

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    A simple, precise and accurate stability indicating RP-HPLC method has been developed and subsequently validated for estimation of Sofosbuvir (SOF) n bulk and from their combination dosage form using a Kromasil C18 (250mm × 4.6 mm, 5µ) at 250C. Mobile phase consisted mixture of 0.1% Ortho phosphoric acid buffer and acetonitrile in the ratio 55:45 (v/v) with flow rate was 1 ml/min and detection was carried out by photodiode array detector at 260nm. The retention time for SOF was found to be 2.06 min. SOF and their dosage form were exposed to thermal, photolytic, oxidative, acid-base hydrolytic stress conditions, the stressed samples were analyzed by proposed method. Peak purity results suggested no other co-eluting, interfering peaks from excipients, impurities, or degradation products due to variable stress condition, and the method is specific for the estimation of SOF in presence of their degradation products and impurities. The proposed method has permitted the quantification of SOF over linearity in the range of 100 – 600 µg/ml and its percentage recovery was found to be 99.10 – 101.74 %. The % RSD of intraday and inter day precision were found 0.3% and 0.6% according to International Conference on Harmonization (ICH) Q2B guidelines.

  2. Stability indicating method development and validation for the estimation of Doxorubicin by using RP-HPLC method in a bulk and pharmaceutical dosage formDownload Article

    T. Rama Mohan Reddy

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    A simple and selective RP-HPLC method is described for the determination of Doxorubicin dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of HPLC water (pH 5.8): ACN (55:45v/v), with detection of 281 nm. Linearity was observed in the range 75-150 µg/ml for Doxorubicin (R2 =0.994) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. The proposed methods were validated. The accuracy of the methods was assessed by recovery studies at three different levels. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form. The developed RP-HPLC and UV spectrophotometric method were successfully applied for the quantitative determination of cited drugs in pharmaceutical dosage form.

  3. LACTOWHEY powder: Provides body’s defense against cancerDownload Article

    Govind Shukla, Nagalakshmi Yaparthy, D.Sruthi Rao, C.J. Sampath Kumar

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    Whey protein concentrates have been researched extensively in the prevention and treatment of cancer. Glutathione stimulation is thought to be the primary immune-modulating mechanism. The amino acid precursors to glutathione available in whey might increase glutathione concentration in relevant tissues,stimulate immunity, and detoxify potential carcinogens. the iron-binding capacity of whey may also contribute to anticancer potential, as iron may act as a mutagenic agent causing oxidative damage to tissues. This article reviews the current available scientific literature regarding the effect of Lactowhey powder in promoting immune function & as a supportive therapy to prevent muscle loss, weight loss and protein calorie malnutrition in cancer patients

  4. The effect of conjugation on different polymers in bioadhesive films of losartanDownload Article

    Ravuri Sandeep, Sivakumar S

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    The objective of the present study was to develop mucoadhesive buccal films of losartan potassium using sodium alginate, chitosan, and their conjugated derivatives at different concentrations by solvent casting technique. Glycerin was used as plasticizer, at different weight ratios. The conjugation of polymers (sodium alginate-cysteine and chitosan-thioglycolic acid) was confirmed by FTIR study. The resulted conjugated sodium alginate and chitosan displayed 475.64±24.31 and 305±31.21 μmol thiol groups per g conjugate, respectively. The developed films were evaluated by different parameters such as weight uniformity, thickness, surface pH, swelling index, in vitro residence time, mucoadhesive strength, in vitro and ex vivo drug permeation. The results indicated that the formulations S5, C4, Cs and Cc showed better characteristic properties and drug permeation. Good results were obtained both in vitro and ex vivo conditions for optimized film. A higher glycerin percent in films increased the drug permeation, particularly at a polymer concentration of 10 to 30% w/v (S2-S4 and C2-C4). A further increase of glycerin (40 to 50% w/v) did not improve losartan potassium permeation (S6 and C6). Formulations Cs and Cc containing conjugated sodium alginate and chitosan resulted in a significantly higher bioadhesion, swelling with better drug permeation over long period than films comprising unmodified polymers. Stability studies on optimized formulations indicated that the formulations are stable as no significant difference was observed in either drug content or drug release.