Design Chromatographic method for Emtricitabine assay using Gradient Phase by RP-HPLC method

Abstract

A stability indicating RP-HPLC method has been developed for quantitative determination of Emtricitabine, in Pharmaceutical dosage forms. Chromatographic separation was attained through gradient elution. Detection wavelength was monitored at 260 nm. The retention times of the Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz was about 4.7min respectively. The developed method was validated as per ICH guidelines. This technique is found to be simple, fast and economical Hence this validated technique method can be used in routine quality testing of individual dosage forms and combination dosage forms of Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz.