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Abstract
A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Lafutidine and Rabeprazole, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Altima C18 (4.6mm x 150mm, 5µm) column using a mixture of ACN, Methanol and Phosphate buffer pH4.6 (10:25:65 v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 265nm. The retention time of the Lafutidine and Rabeprazole was 2.088, 6.068 ±0.02min respectively. The method produces linear responses in the concentration range of 10-50mg/ml of Lafutidine and 20-100mg/ml of Rabeprazole. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.