Main Article Content


Aim of the present research work was to develop a sensitive, precise and robust UPLC method for the simultaneous estimation of tipiracil and trifluridine in formulations. The chromatographic separation of mixture of tipiracil and trifluridinewas attained in isocratic method utilizing a mobile phase of 0.1% ortho phosphoric acid: acetonitrile in the proportion of 50:50%v/v utilizing a Hibera C18 column which has dimensions of 100 x 2.1 mm, 1.8mparticle size and the flow rate of 1.0 ml/min. The detection system was monitored at 230nm wavelength maximum with 1.5 ml injection volume. The retaining time for trifluridine and tipiracilwas achieved at 0.549min, and 1.251 min respectively. The developed method was highly sensitive, rapid, precise and accurate than the earlier reported methods. The total run time was decreased to 3.0 min; hence, the technique was more precise and economical. The projected method can be utilized for routine analysis in quality control department in Pharma industries.


Tipiracil Trifluridine Isocratic elution UPLC

Article Details