Method development and validation for simultaneous estimation of Fosnetupitant and Palonosetron by RP-HPLC

Authors

  • Arshiya Department of Pharmaceutical Analysis, Shadan Women’s College of Pharmacy, Khairatabad, Hyderabad, India
  • Ayesha Begum K Department of Pharmaceutical Analysis, Shadan Women’s College of Pharmacy, Khairatabad, Hyderabad, India

Keywords:

fosnetupitant, palonosetron, HPLC, Method development, Validation

Abstract

A simple and precise method was developed for estimating fosnetupitant as well as palonosetron. The method was found to be specific and precise. The separation was attained on Xterra RP18 column and linearity was achieved in the concentration rage of 117µg/mL to 470µg/mL of Fosnetupitant, 0.125µg/mL to 0.5µg/mL of Palonosetron with correlation coefficient 0.99. The percent recovery from the assay was found to be 100.19%for fosnetupitant and 100.30% for palonosetron. Limit of detection and quantitation for fosnetupitant and palonosetron were within the acceptable range. From the stability studies, the percentage variation was less than 10.0% which is the desired criteria. Therefore, this method can be adopted to estimate fosnetupitant as well as palonosetron in other pharmaceutical formulations.

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Published

2021-10-28

Issue

Vol. 6 No. 3 (2020): Vol 6, No. 3 (2020) 2020 Volume - 6 Issue-3

Section

Articles

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