Evaluation of novel methotrexate nanoparticles for anticancer action

Authors

  • Amaresha S Department of Pharmacy, JJT University, Jhunjhunu, Rajasthan, India

Keywords:

Invitro, Invivo anticancer activities, Methotrexate nanoparticles and toxicity studies

Abstract

Methotrexate-chitosan nanoparticles (MTX-NP) were prepared by method of Tripolyphosphate (TPP) ionic cross- linking. Influence of preparation conditions on physicochemical properties were analyzed by DLS, SEM & FTIR. In- vitro drug release properties were investigated by UV–VIS spectroscopy. In vitro anti-cancerous activities (MTT assay, apoptosis assay, cell cycle arrest & ROS indices) of MTX/MTX-NP were evaluated on HT-1080 & MCF-7 cells. Comparative (MTX/MTX-NP) in vivo pharmacokinetic & Acute/sub acute toxicity studies carried out on Wistar rats. In conclusion, prepared MTX-NP has played significant role in increasing in vitro-anti cancer efficacy, in vivo pharmacokinetic profiles & as well helps in reducing toxic effect.

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Published

2021-11-12

Issue

Vol. 5 No. 4 (2019): Vol 5, No. 4 (2019) 2019 Volume - 5 Issue-4

Section

Articles

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