Validated Spectrophotometric Quantitation of Tenofovir disoproxil fumarate in Bulk and Tablet Dosage Form

Authors

  • Pooja M Department of Pharmaceutical Chemistry, Ikon Pharmacy college, Bheemanahalli, Bidadi, Ramanagra Taluk and District, Karnataka, India – 562 109
  • Yogesh M Department of Pharmaceutical Chemistry, Ikon Pharmacy college, Bheemanahalli, Bidadi, Ramanagra Taluk and District, Karnataka, India – 562 109

Keywords:

Tenofovir disoproxil fumarate, Zero order derivative Spectroscopy, Ethanol, Accuracy

Abstract

A novel, simple, accurate and precise Zero order derivative spectroscopic method was developed and validated for the estimation of TDF in bulk and Pharmaceutical dosage forms and has an absorption maximum at 260 nm in Ethanol. The Linearity was found to be in the concentration range of 5-30μg/ml and the correlation coefficient was found to be 0.9994 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y = 0.0305 X + 0.0009. The % recovery values were found to be within 99.70 -100.62 % showed that the method was accurate. The LOD and LOQ were found to be 0.120 and 0.362 μg/ml, respectively. The % RSD values were less than 2. The method has been validated according to ICH guidelines for linearity, accuracy, precision, robustness, ruggedness. Limit of detection and limit of quantitation. Proposed method was successfully applied for the quantitative estimation of TDF in bulk and pharmaceutical dosage form.

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Published

2021-12-21

Issue

Vol. 7 No. 4 (2021): Vol 7, No. 4 (2021) 2021 Volume - 7 Issue-4

Section

Articles

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